Sequencing
DNA sequencing services are currently provided using the Applied Biosystems 3730xl DNA Analyzer. The ABI machines utilize a one-capillary cycle sequencing process with refined, four-dye fluorescent labeling methods and a real-time scanning detector. The biochemistry of one-lane sequencing is similar to Sanger-based methods.
The JHU Finch server automatically processes data after electrophoresis is complete and provides quality assessment for each sample. Data is available for download, viewing, and editing.
The service provides standard sequencing primers for several vectors. Custom primers are provided by the user or can be synthesized in our facility.
There are three deadlines for drop off of samples each day. Standard daily schedules require samples to be dropped off in Blalock by 1 PM or 3 PM. 1PM samples will be ready by 10 AM the following day, 3 PM samples will be available the following afternoon. Same day sequencing service is available at a higher fee per sample, with a minimum of 5 samples. Samples must be ordered on the JHU Finch server and physically present in our facility by 9 AM and data will be released around 5 PM.
Drop off times for the remote locations are as follows:
CRB II Room LB06: Samples dropped off here by 2 PM will normally be picked up and processed the same day, making the data available the following afternoon.Bayview Campus: Drop off in the Core 24/7 on the first floor of the Asthma and Allergy building, in slot F51. Pickup is once daily. Samples dropped off by 11:45 AM will have data available by 10 AM the next morning. Samples dropped off after 11:45 will wait for the next day.